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CABRI:ICLC Accession_number ICLC HTL97013 Cell_line_name A-172 Brief_description Species: human; Tumor: glioblastoma Description Species: human male 53 years old; Tumor: glioblastoma Depositor Obtained from ECACC. Bibliographic_reference J Natl Cancer Inst 1973;51:1417-1423 [PMID: 4357758 ] Morphology glial, grown as monolayer Culture_conditions continuous culture; DMEM + 10% FBS + 2mM L-Glutamine; Seed at 2-4x104 cells/cm2; split confluent cultures 1:4-1:8 using trypsin/EDTA. 37C, 5% CO2. Viruses Search for viruses was not performed Release_conditions Cell line available for distribution. For non-commercial investigative use only Hazard - Passage_number 318 Species_validation Validated by isoenzymes: confirmed as human with G6PD, NP Tumorigenicity non tumorigenic in anti thymocyte Karyology hypertriploid; n = 80, over 20 marker chromosomes per cell Freezing_medium Culture medium + 50% FBS + 10% DMSO Sterility mycoplasma negative, HOECHST and PCR

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